Over Expression:Article Title: Cardioprotective function of sclerostin by reducing calcium deposition, proliferation, and apoptosis in human vascular smooth muscle cells
Article Snippet: .. Overexpression of sclerostin was carried out by producing a second-generation lentiviral packaging system protocol using the vectors pVSV-G (Addgene), that expresses the envelope gene of the VSV-G virus, psPAX2 (Addgene) that expresses the reverse transcriptase gene, the protease gene and the gene for assembly of the HIV-1 virus and, the pLVX: SOST construct or empty pLVX (Addgene). .. HEK293T cells were transfected with the mix of the above plasmids using polyethylenimine (Quimigen) and were cultured in DMEM/F-12 GlutaMAX (Gibco) supplemented with 10% FBS and grown under standard conditions for 24 h. Lentivirus particles were harvested, filtered, ultracentrifuged, resuspended in PBS and stored at -80oC.
Article Title: Cardioprotective function of sclerostin by reducing calcium deposition, proliferation, and apoptosis in human vascular smooth muscle cells.
Article Snippet: .. Second-generation lentiviruses and transduction for generation of stable lines of HAoSMCs Overexpression of sclerostin was carried out by producing a second-generation lentiviral packaging system protocol using the vectors pVSV-G (Addgene), that expresses the envelope gene of the VSV-G virus, psPAX2 (Addgene) that expresses the reverse transcriptase gene, the protease gene and the gene for assembly of the HIV-1 virus and, the pLVX:SOST construct or empty pLVX (Addgene). .. HEK293T cells were transfected with the mix of the above plasmids using polyethylenimine (Quimigen) and were cultured in DMEM/F-12 GlutaMAX (Gibco) supplemented with 10% FBS and grown under standard conditions for 24 h. Lentivirus particles were harvested, filtered, ultracentrifuged, resuspended in PBS and stored at -80oC.
Virus:Article Title: Cardioprotective function of sclerostin by reducing calcium deposition, proliferation, and apoptosis in human vascular smooth muscle cells
Article Snippet: .. Overexpression of sclerostin was carried out by producing a second-generation lentiviral packaging system protocol using the vectors pVSV-G (Addgene), that expresses the envelope gene of the VSV-G virus, psPAX2 (Addgene) that expresses the reverse transcriptase gene, the protease gene and the gene for assembly of the HIV-1 virus and, the pLVX: SOST construct or empty pLVX (Addgene). .. HEK293T cells were transfected with the mix of the above plasmids using polyethylenimine (Quimigen) and were cultured in DMEM/F-12 GlutaMAX (Gibco) supplemented with 10% FBS and grown under standard conditions for 24 h. Lentivirus particles were harvested, filtered, ultracentrifuged, resuspended in PBS and stored at -80oC.
Article Title: Cardioprotective function of sclerostin by reducing calcium deposition, proliferation, and apoptosis in human vascular smooth muscle cells.
Article Snippet: .. Second-generation lentiviruses and transduction for generation of stable lines of HAoSMCs Overexpression of sclerostin was carried out by producing a second-generation lentiviral packaging system protocol using the vectors pVSV-G (Addgene), that expresses the envelope gene of the VSV-G virus, psPAX2 (Addgene) that expresses the reverse transcriptase gene, the protease gene and the gene for assembly of the HIV-1 virus and, the pLVX:SOST construct or empty pLVX (Addgene). .. HEK293T cells were transfected with the mix of the above plasmids using polyethylenimine (Quimigen) and were cultured in DMEM/F-12 GlutaMAX (Gibco) supplemented with 10% FBS and grown under standard conditions for 24 h. Lentivirus particles were harvested, filtered, ultracentrifuged, resuspended in PBS and stored at -80oC.
Reverse Transcription:Article Title: Cardioprotective function of sclerostin by reducing calcium deposition, proliferation, and apoptosis in human vascular smooth muscle cells
Article Snippet: .. Overexpression of sclerostin was carried out by producing a second-generation lentiviral packaging system protocol using the vectors pVSV-G (Addgene), that expresses the envelope gene of the VSV-G virus, psPAX2 (Addgene) that expresses the reverse transcriptase gene, the protease gene and the gene for assembly of the HIV-1 virus and, the pLVX: SOST construct or empty pLVX (Addgene). .. HEK293T cells were transfected with the mix of the above plasmids using polyethylenimine (Quimigen) and were cultured in DMEM/F-12 GlutaMAX (Gibco) supplemented with 10% FBS and grown under standard conditions for 24 h. Lentivirus particles were harvested, filtered, ultracentrifuged, resuspended in PBS and stored at -80oC.
Article Title: Cardioprotective function of sclerostin by reducing calcium deposition, proliferation, and apoptosis in human vascular smooth muscle cells.
Article Snippet: .. Second-generation lentiviruses and transduction for generation of stable lines of HAoSMCs Overexpression of sclerostin was carried out by producing a second-generation lentiviral packaging system protocol using the vectors pVSV-G (Addgene), that expresses the envelope gene of the VSV-G virus, psPAX2 (Addgene) that expresses the reverse transcriptase gene, the protease gene and the gene for assembly of the HIV-1 virus and, the pLVX:SOST construct or empty pLVX (Addgene). .. HEK293T cells were transfected with the mix of the above plasmids using polyethylenimine (Quimigen) and were cultured in DMEM/F-12 GlutaMAX (Gibco) supplemented with 10% FBS and grown under standard conditions for 24 h. Lentivirus particles were harvested, filtered, ultracentrifuged, resuspended in PBS and stored at -80oC.
Construct:Article Title: Cardioprotective function of sclerostin by reducing calcium deposition, proliferation, and apoptosis in human vascular smooth muscle cells
Article Snippet: .. Overexpression of sclerostin was carried out by producing a second-generation lentiviral packaging system protocol using the vectors pVSV-G (Addgene), that expresses the envelope gene of the VSV-G virus, psPAX2 (Addgene) that expresses the reverse transcriptase gene, the protease gene and the gene for assembly of the HIV-1 virus and, the pLVX: SOST construct or empty pLVX (Addgene). .. HEK293T cells were transfected with the mix of the above plasmids using polyethylenimine (Quimigen) and were cultured in DMEM/F-12 GlutaMAX (Gibco) supplemented with 10% FBS and grown under standard conditions for 24 h. Lentivirus particles were harvested, filtered, ultracentrifuged, resuspended in PBS and stored at -80oC.
Article Title: Cardioprotective function of sclerostin by reducing calcium deposition, proliferation, and apoptosis in human vascular smooth muscle cells.
Article Snippet: .. Second-generation lentiviruses and transduction for generation of stable lines of HAoSMCs Overexpression of sclerostin was carried out by producing a second-generation lentiviral packaging system protocol using the vectors pVSV-G (Addgene), that expresses the envelope gene of the VSV-G virus, psPAX2 (Addgene) that expresses the reverse transcriptase gene, the protease gene and the gene for assembly of the HIV-1 virus and, the pLVX:SOST construct or empty pLVX (Addgene). .. HEK293T cells were transfected with the mix of the above plasmids using polyethylenimine (Quimigen) and were cultured in DMEM/F-12 GlutaMAX (Gibco) supplemented with 10% FBS and grown under standard conditions for 24 h. Lentivirus particles were harvested, filtered, ultracentrifuged, resuspended in PBS and stored at -80oC.
Transduction:Article Title: Cardioprotective function of sclerostin by reducing calcium deposition, proliferation, and apoptosis in human vascular smooth muscle cells.
Article Snippet: .. Second-generation lentiviruses and transduction for generation of stable lines of HAoSMCs Overexpression of sclerostin was carried out by producing a second-generation lentiviral packaging system protocol using the vectors pVSV-G (Addgene), that expresses the envelope gene of the VSV-G virus, psPAX2 (Addgene) that expresses the reverse transcriptase gene, the protease gene and the gene for assembly of the HIV-1 virus and, the pLVX:SOST construct or empty pLVX (Addgene). .. HEK293T cells were transfected with the mix of the above plasmids using polyethylenimine (Quimigen) and were cultured in DMEM/F-12 GlutaMAX (Gibco) supplemented with 10% FBS and grown under standard conditions for 24 h. Lentivirus particles were harvested, filtered, ultracentrifuged, resuspended in PBS and stored at -80oC.
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